No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Can we do the ligand-protein simulation using Charmm27 in gromacs2020.1? if so, then how?

The ligand.str file generated using cgenff is only compatible with charmm36. Any suggestions on how i can convert this str file into the gromacs topology file using Charmm27 ff?

07 January 2021 8,706 1 View

Cell line that does not express any of the VEGF receptors?

I am looking for VEGFR-negative cell lines, that either show very low or no expression at all of VEGF receptors for cell signalling assays.

01 November 2020 6,046 1 View

Is there a way to correlate one-dimensional netcdf data (like a time-series data) with the spatial gridded dataset (which is multi-dimensional)?

For eg. I have a netcdf file which is in the form of a time-series and the other data is a gridded .nc file for geopotential height. I want to correlate both of these datasets. Please guide me...

27 May 2020 8,133 3 View

I have doped ZnO nanoparticles using tungsten and antimony.1% doping..their atomic radius is different so doping would be possible or not?

i have analyzed samples using XRD, i got peaks with a slight peak shift for pure and doped material. no any addition peak was found. just XRD is enough to confirm the doping?

04 December 2019 2,030 3 View

I am working on a medical imaging research and if anyone knows where I could find CT scans of the brain with and without contrast agents?

I have looked up Cancer Imaging Archive but I need a bigger dataset. Can anyone help me out with some good places to look at for more images with and without contrast agents?

28 October 2019 4,219 3 View

Extracting smart health band data?

We need to download or extract health data such as heart rate, b.p, calories burned etc. from smart health-band. However, most of the bands are associated with their makers' app and do not provide...

03 June 2019 3,655 1 View

Can transwell assays be used to study protein transfer between two discrete populations of cells?

Hello, I was wondering if transwell assays with permeable supports could be used to measure protein transfer between two different populations of cells. If I used a small pore size (less than 3...

05 November 2018 8,364 1 View

Does the nature of ligand affect the crystallization process or rate of nucleation during the crystal growth ?

my ligand is completely soluble in DMSO but very less soluble in other organic solvents.. I tried many times to obtain the crystals of my ligand with mercury using cobinations of solvents like...

27 August 2018 7,971 15 View

I need help on deciding which journal to submit my publication?

Hello I am graduate student and need help deciding which journal to submit my article. The article pertains to surface and inter-facial properties of food oils. It is very engineering and physical...

05 March 2018 4,071 8 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View