No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

Can we do the ligand-protein simulation using Charmm27 in gromacs2020.1? if so, then how?

The ligand.str file generated using cgenff is only compatible with charmm36. Any suggestions on how i can convert this str file into the gromacs topology file using Charmm27 ff?

07 January 2021 8,706 1 View

Cell line that does not express any of the VEGF receptors?

I am looking for VEGFR-negative cell lines, that either show very low or no expression at all of VEGF receptors for cell signalling assays.

01 November 2020 6,046 1 View

Is there a way to correlate one-dimensional netcdf data (like a time-series data) with the spatial gridded dataset (which is multi-dimensional)?

For eg. I have a netcdf file which is in the form of a time-series and the other data is a gridded .nc file for geopotential height. I want to correlate both of these datasets. Please guide me...

27 May 2020 8,133 3 View

I have doped ZnO nanoparticles using tungsten and antimony.1% doping..their atomic radius is different so doping would be possible or not?

i have analyzed samples using XRD, i got peaks with a slight peak shift for pure and doped material. no any addition peak was found. just XRD is enough to confirm the doping?

04 December 2019 2,030 3 View

I am working on a medical imaging research and if anyone knows where I could find CT scans of the brain with and without contrast agents?

I have looked up Cancer Imaging Archive but I need a bigger dataset. Can anyone help me out with some good places to look at for more images with and without contrast agents?

28 October 2019 4,219 3 View

Extracting smart health band data?

We need to download or extract health data such as heart rate, b.p, calories burned etc. from smart health-band. However, most of the bands are associated with their makers' app and do not provide...

03 June 2019 3,655 1 View

Can transwell assays be used to study protein transfer between two discrete populations of cells?

Hello, I was wondering if transwell assays with permeable supports could be used to measure protein transfer between two different populations of cells. If I used a small pore size (less than 3...

05 November 2018 8,364 1 View

Does the nature of ligand affect the crystallization process or rate of nucleation during the crystal growth ?

my ligand is completely soluble in DMSO but very less soluble in other organic solvents.. I tried many times to obtain the crystals of my ligand with mercury using cobinations of solvents like...

27 August 2018 7,971 15 View

I need help on deciding which journal to submit my publication?

Hello I am graduate student and need help deciding which journal to submit my article. The article pertains to surface and inter-facial properties of food oils. It is very engineering and physical...

05 March 2018 4,071 8 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

What is the right way to conjugate two acids?

How to conjugate two acids with amine linker?

18 February 2021 4,083 2 View

What is error #2070 in gaussian.how we do resolve it?

We want to ONIOM calculation in gaussian16. However, gaussian doesn't work. What should we do? Atom 15 layer R linked to atom 85 is also bonded to atom 161 in layer M bad...

15 February 2021 7,766 3 View