What volume of Phenol chloroform I should add for phenol chloroform extraction ,if my sample volume is 1ml?

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Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

Can anyone confirm the bench mark value of Effect Size (ƒ2)?

Effect sizes are evaluated as small (0.02), medium (0.15) or large (0.35) respectively, according to Cohen (1988). OR According to Cohen (1988; 2019), the f2 values of 0.10 represent small effect;...

29 June 2024 830 1 View

How to control the shape and orientation of MoS2/MoSe2 Growth in CVD?

During MoSe2 growth in CVD, which factor influences most of on shape of the crystal .. I see different shapes like stars, triangles, and polygonal. etc Secondly, which parameter can influence to...

04 June 2024 1,027 1 View

How to coat particles with Graphene Oxide?

Is it possible to coat Si or SiO particles with Graphene Oxide dispersed in DI water by stir drying technique?

08 May 2024 6,307 3 View

Stress Relaxation Curve from Hyperelastic Model in ABAQUS?

Hi, I am trying to get stress relaxation profile from a simple hyperelastic model. Step: I applied 2 steps(static, geometry) in a simple 3D cylindrical model where first step was 30 secs and...

19 April 2024 3,128 0 View

Discrepancy in Cytotoxicity Assessment of MDA-MB231 Cell Line: Seeking Clarification on IC50 Determination?

I'm currently conducting an experiment on the MDA-MB231 cell line, aiming to assess cytotoxicity using the XTT assay to determine the IC50. Initially, I seeded the cells at a density of 7000 cells...

14 April 2024 1,882 1 View

Hello! I want to do the all antioxidant activity but the reagent show no blue color with ascorbic acid dilutions. Why?

The reagent made of (ammonium heptamolybdate, sodium phosphate and sulphuric acid) made up to the volume of 100 mL by deionized water does not change to blue color when added in ascorbic acid...

24 March 2024 1,652 0 View

Inquiry Regarding Preservation and Revival of MDA-MB231 Cell Line?

Dear Research Community, I am writing to seek insights and advice regarding an issue encountered with the revival of the MDAMB231 cell line. Approximately one month ago, I preserved the MDAMB231...

23 March 2024 8,774 2 View

Chi-Square large difference in Invariance Test via AMOS?

Dear Experts, I need your guidance on the invariance test performed via AMOS. My friend is comparing two groups, A and B. Their Model fits are excellent, less than 3. CFI is 0.98 for both groups....

12 March 2024 9,333 5 View

Ph.D in Cyber Security?

Please inform me about universities who offer online Ph.D in Cyber Security area with little or minimum fee, as I want to pursue the study but due to financial problems facing difficulties. Your...

21 February 2024 9,874 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View