What volume of Phenol chloroform I should add for phenol chloroform extraction ,if my sample volume is 1ml?

My lysate volume after ultracentrifugation is 1ml(after resuspension of pellet),but the protocols I have read are about 450-500ul volumes of lysate.What volume of P:C i should use for 1 ml volume?

16 April 2018 1,454 2 View