I'm currently conducting an experiment on the MDA-MB231 cell line, aiming to assess cytotoxicity using the XTT assay to determine the IC50. Initially, I seeded the cells at a density of 7000 cells per well and treated them with varying concentrations of the drug ranging from 0.1 to 40 micromolar after 48 hours of seeding. Surprisingly, even after treatment with 40 micromolar, the observed cytotoxicity was not significant, with approximately 20% of cells exhibiting cell death.
However, it has come to my attention that a fellow researcher conducted a similar experiment on the same cell line using the same drug. Remarkably, they reported an IC50 range between 20 to 35 micromolar.
I am confused by this discrepancy and would greatly appreciate any insights or suggestions regarding potential factors that could contribute to such inconsistencies in cytotoxicity assessment.
Thank you.
It would be helpful if you could specify the drug you are researching.
The issue might be quite simple. It's possible the stock drug has degraded and lost its effectiveness what affected all dilutions. Additionally, there could have been errors in your calculations or when preparing the dilutions. Have you attempted to repeat the experiment using freshly prepared dilutions?
- Badges
- Science topic
- Similar topics
- Filing