What tools can I use to generate good quality Multiple sequence alignment images like the one given below?

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Chandra Sekar Ponnusamy

Dear Sakshi,

After generating alignment file in any of the online MSA servers like ClustalOmega upload the same in ESPRIP tool.

Link:

https://espript.ibcp.fr/ESPript/ESPript/

I have attached a sample output file for your reference.

FYI:

ESPript, 'Easy Sequencing in PostScript', is a program which renders sequence similarities and secondary structure information from aligned sequences for analysis and publication purpose.

Abhijeet Singh

This image is not generated through any software. It is a manually annotated image. You can do it is any editor software like GIMP, Inkscape, Photoshop or most easy Libreoffice impress / MS Powerpoint.

If you want to do this via a more complicated, try UniPro UGENE (http://ugene.net/), PFAAT (https://pfaat.sourceforge.io/).

Karthik Narayan

I agree, when you work with high data, programming in R is lot easy and quicker. For me big data gets struck in MEGA

Sawar Khan

Try with SnapGene