I am interested in isolating phages from environmental samples and different papers suggest different methods to do so. Many people use SM Buffer and phage buffer to re-suspend the isolated virus particles. Are these universal buffers for phage? Are there other types?
I notice the components of the buffers include salts such as MgSO4, CaCl2 and NaCl2. I know that CaCl2 is required for adsorption for P1 phage. What about the other salts, are they also required for adsorption for other types of phages? Are there other types of salts that are not present in SM/phage buffer that could be essential for phage isolation too?
Should these salts be added in the agar/broth as well?
Any help is appreciated!
I'm not sure if it is a universal buffer but the SM-buffer is a well standarized buffer for phage storage. From my own experience, I noticed an improvement of phage isolation and/or enrichment when I added CaCl2 and MgSO4 to the broth. There is indeed evidence that these salts enhance the attachment of the phages to the bacterial hosts ( C. Carvalho et al 2009; Aarestrup et al. 2007; Salama et al. 1989).
Hi, Mgcl2 is also helpful. I have noticed that salt can only help phages during attachment to their host which may enhance your enrichment. So there is no need for additional salt once you isolate your phage. Phage is highly dependent on their host so you may need to enhance your enrichment method.
Hi
The studies to be reported the only inorganic salts used were CaCl, or MgSO4.
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