Could anyone give me help. I sent a homogenized sample from ducks to a Bioanalyzer unite to make a quality assurance test prior to small RNA seq. Some of the samples came without RIN. Some of them show a smeared Band. Others with a RIN of 2.3.
Actually I sent 300ng/µl of RNA and the person in the unite diluted the sample 1:10 and he used a Pico chip for the analysis. What might be the cause of this? Do you think that this results can be suitable for small RNA seq knowing that these small RNAs are more stable.
@Mathilde Cordellier, this is what happens to me:
I have great amounts and purities of my RNAs but I get no RIN, it seems to be undetectable even if it should not. I see no bands (or very faint) in the electophoresis, so RNAs are not degraded.
If I try to modify the parameters? Do u think it could help me?
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