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Hello to everyone. I have a question about the RNA extraction by using Trizol. Almost everytime that I do this protocol, I obtain a very bad 260/230. This happens even if I have a good RNA...
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Help!!!!!!! Does anyone have experience with PlatE cells for the production of virus? Thank you very much!!!!
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Hello to everyone! I want to see little proteins (15-20 kDa) by WB and for the transfer I have the semi-dry transfer blot Biorad. Generally, for a 10% gel I transfer at 80mA per gel for 1h and the...
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