Dear all,
Any comment on the reasons of appearance of notched qPCR curves ? See attached. The machine in Roch LC480
Could You help explaning the reasons why a notched curve after qPCR appears ? The machine is Roch
09 October 2018 6,108 0 View
I have a list of miRNAs and a list of predicted target mRNAs. The mRNA targets were created by other ways. I am searching for a tool to test whether an interaction (binding) could exist between...
08 September 2018 6,275 4 View
Dear All, Could any body help me in finding the best statistical/model to see the un-measured titres of virus in a time points that extend beyond the ones I measured. For instance I am testing...
07 August 2018 3,896 2 View
I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...
04 May 2018 1,138 6 View
What is the suitable regression to be used if I have binary independent variables and also the dependent variables ar binary ? i.e. yes/no ? Details: I am checking whether the presence/absence of...
09 October 2017 777 6 View
Dear All, Could any of you suggest how to get to the function of small nucleolar RNAs ? I got tons of them after a small RNA sequencing experiment and would like to know their functions or...
08 September 2015 9,231 1 View
I have a time series experiment and want to compare DE miRNAs between these time points and the Control. I realized that I loos some DE miRNAs upon combining some time points together and conduct...
08 September 2015 1,591 1 View
I am searching for miRNA annotated for duck and turkey. Any help
04 May 2015 1,060 2 View
Can I use U6 primer assay from Qiagen as a standard gene in miRNA detection in chicken samples ? Both are 100 % conserved ? Also Can I use hsa-miR-155 primer for detection of gga-miR-155 if they...
04 May 2015 3,390 1 View
My question in concerned with miRNAs in-situe detection. I have 3 separate cell line, and want to prove the idea that certain miRNAs are expressed upon influenza in one cell type but not in the...
10 November 2014 9,247 1 View
I want to know more about Uranium ore deposits in world.
11 August 2024 6,720 0 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
Thanks
03 August 2024 3,567 2 View
En suivant toutes les étapes mentionnées sur (ajouter une recherche) il me signale erreur, ressayer, j'ai essayé plusieurs fois, sans succès, toujours il me signal erreur, ressayer
02 August 2024 9,719 1 View
I want to know more about diamond ore deposits in Iran.
02 August 2024 7,399 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
La politique de financement est en soit une politique publique. Comment les évaluer dans un travail de recherche scientifique ?
22 July 2024 6,495 2 View