Could You help explaning the reasons why a notched curve after qPCR appears ? The machine is Roch
Dear all, Any comment on the reasons of appearance of notched qPCR curves ? See attached. The machine in Roch LC480
09 October 2018 2,110 0 View
I have a list of miRNAs and a list of predicted target mRNAs. The mRNA targets were created by other ways. I am searching for a tool to test whether an interaction (binding) could exist between...
08 September 2018 6,275 4 View
Dear All, Could any body help me in finding the best statistical/model to see the un-measured titres of virus in a time points that extend beyond the ones I measured. For instance I am testing...
07 August 2018 3,896 2 View
I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...
04 May 2018 1,138 6 View
What is the suitable regression to be used if I have binary independent variables and also the dependent variables ar binary ? i.e. yes/no ? Details: I am checking whether the presence/absence of...
09 October 2017 777 6 View
Dear All, Could any of you suggest how to get to the function of small nucleolar RNAs ? I got tons of them after a small RNA sequencing experiment and would like to know their functions or...
08 September 2015 9,231 1 View
I have a time series experiment and want to compare DE miRNAs between these time points and the Control. I realized that I loos some DE miRNAs upon combining some time points together and conduct...
08 September 2015 1,591 1 View
I am searching for miRNA annotated for duck and turkey. Any help
04 May 2015 1,060 2 View
Can I use U6 primer assay from Qiagen as a standard gene in miRNA detection in chicken samples ? Both are 100 % conserved ? Also Can I use hsa-miR-155 primer for detection of gga-miR-155 if they...
04 May 2015 3,390 1 View
My question in concerned with miRNAs in-situe detection. I have 3 separate cell line, and want to prove the idea that certain miRNAs are expressed upon influenza in one cell type but not in the...
10 November 2014 9,247 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View