22 Questions 54 Answers 0 Followers
Questions related from Mohamed Samir
Could You help explaning the reasons why a notched curve after qPCR appears ? The machine is Roch
10 October 2018 6,076 0 View
Dear all, Any comment on the reasons of appearance of notched qPCR curves ? See attached. The machine in Roch LC480
10 October 2018 2,043 0 View
I have a list of miRNAs and a list of predicted target mRNAs. The mRNA targets were created by other ways. I am searching for a tool to test whether an interaction (binding) could exist between...
09 September 2018 6,234 4 View
Dear All, Could any body help me in finding the best statistical/model to see the un-measured titres of virus in a time points that extend beyond the ones I measured. For instance I am testing...
08 August 2018 3,845 2 View
I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...
05 May 2018 1,086 6 View
What is the suitable regression to be used if I have binary independent variables and also the dependent variables ar binary ? i.e. yes/no ? Details: I am checking whether the presence/absence of...
10 October 2017 728 6 View
Dear All, Could any of you suggest how to get to the function of small nucleolar RNAs ? I got tons of them after a small RNA sequencing experiment and would like to know their functions or...
09 September 2015 9,179 1 View
I have a time series experiment and want to compare DE miRNAs between these time points and the Control. I realized that I loos some DE miRNAs upon combining some time points together and conduct...
09 September 2015 1,552 1 View
I am searching for miRNA annotated for duck and turkey. Any help
05 May 2015 1,029 2 View
Can I use U6 primer assay from Qiagen as a standard gene in miRNA detection in chicken samples ? Both are 100 % conserved ? Also Can I use hsa-miR-155 primer for detection of gga-miR-155 if they...
05 May 2015 3,355 1 View
My question in concerned with miRNAs in-situe detection. I have 3 separate cell line, and want to prove the idea that certain miRNAs are expressed upon influenza in one cell type but not in the...
11 November 2014 9,220 1 View
I am doing SYBR green based qPCR for HA gene of influenza. I have two questions: 1. I did a challenge experiment twice and in the first time, my primer (amplify 120bp) worked nicely in different...
10 October 2014 1,917 5 View
Could anyone suggest a way or software or online database to see how conservative miRNAs individual between human and mice ?
10 October 2014 4,080 4 View
Some times I got a positive signals ( Ct around 37) in the NTC in a SYBR green based real time PCR run. I am amplifying gene for Influenza virus. I still have these signals even in a low...
05 May 2014 401 34 View
Some of my colleagues infected ducks with H5N1 virus, but they collected organs from one animal per time point. This means that we do not have biological replicate. How can I measure relatively...
04 April 2014 4,798 3 View
My question is regarding the design of time course experiment. I wanted to know the expression level of one miRNAs in H1N1 infected A549 Cells at 24, 48, 72 hrs post infection and to compare it...
02 February 2014 1,649 7 View
Could anyone give me help. I sent a homogenized sample from ducks to a Bioanalyzer unite to make a quality assurance test prior to small RNA seq. Some of the samples came without RIN. Some of...
02 February 2014 1,197 2 View
I wonder if I infected two cells with IAV using two different MOI, one of them 1 and the other is 10. Now I wanted to compare the expression level of one gene in both cells. I think this is not...
02 February 2014 5,214 6 View
I am going to compare miRNA profiling of different cells infected with H1N1 virus. I will fix them using a MOI of 10. Is there a problem using a high MOI, if I use it in all experiments?
01 January 2014 9,642 3 View
hi all, how to tell R that the row names is for instance certain column, when exporting files to r using read.csv file function ?
01 January 1970 1,382 6 View
Dear All, I have 500 miRNAs with expressed read counts in 20 conditions. In the same 20 conditions, I have measurments of lymphocyte counts. I would like to see how the miRNAs counts are...
01 January 1970 1,643 7 View
Dear All, I have data about the expression of genes (binary data being present or absent by PCR) in 500 isolate of certain bacteria from different reservoirs (e.g. humans, cattle, sheep, and...
01 January 1970 9,832 5 View
