Hi everybody!
In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know the best proportions of each enzyme in my enzymatic mix but I have some problems here:
1) Both enzymes use different concentrations units (cellulase uses percentage and alginate lyase, enzymatic units). If i convert the last one to percentage this value will be very low compared with cellulase.
2) Using ratios instead of proportions is also an option, but in this case I only have two components (and obviously 1 ratio). Is it possible to perform a mixture design using only 1 ratio?
Many thanks in advance
Instead of obtaining protoplast, can you ever tried the use of germinating conidia? It could be more standardized instead of the use of some misleading enzyme reactions.
DOI 10.1007/s10295-010-0912-5
You can use mixture design to determine the optimal mixture proportion for your enzymes. That the enzymes have different units should not be a problem. Just take varying volumes of each enzyme from their stock solutions and use these different volumes as design points in your experimental design. You may choose to use simplex lattice or simplex centroid design. Please note that the total volume of enzymes (cellulase + alginate lyase) must be constant at all design points. Minitab can do this for you effectively. The software also has a very useful guide in its tutorial section.
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