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Questions related from Mushafau Adebayo Oke
I need a simple protocol for the assay of LPMO enzymes using biochemical assays just like those of cellulases. Most of those I have seen in the literature used mass spectrometry. I would be...
07 July 2017 8,498 3 View
Sometimes I get negative OD readings (at 540 nm) when determining reducing sugars using glucose as standard (Miller, 1959). When I use Microsoft Excel to plot the glucose standard curve, the line...
04 April 2015 4,708 15 View
Can anyone provide possible reasons why the specific activity of my cellulase (from bacteria) keeps reducing after each step of purification? So far, the crude enzyme has the highest activity...
12 December 2014 9,284 34 View
The use of HPLC might give some unknown peaks. However, LCMS would allow a more detailed identification of the compounds in the hydrolysate. Such compounds might include sugars (monomers and...
08 August 2014 5,483 4 View
I am trying to precipitate a bacterial cellulase produced on a lignocellulosic hydrolysate as the first step in the purification of the enzyme. I did step-wise ammonium sulphate fractionation at...
08 August 2014 4,432 18 View
One of the most cited references regarding cellulase (saccharifying cellulase, endo-, exo- and betaglucosidase) assays (Ghose, 1987 & Wood and Bhat, 1988) recommend that OD readings of enzyme...
04 April 2014 6,244 1 View
A bacterium was identified by BIOLOG as a particular species; running a BLAST search on the 16S rRNA sequence of the same organism gave a different specie of the same genus. The one with the...
01 January 2014 1,806 28 View
The IUPAC procedure for determining total cellulase activity using filter paper states that one should prepare two dilutions of the enzyme such that one dilution will release > 2 mg glucose from...
11 November 2013 3,210 5 View
I wish to know how to perform life cycle analysis or assessment (LCA) of any biotechnological processes especially for biofuels. What are the basic things to consider? Is there any book or...
09 September 2013 7,958 3 View
In running an experiment to plot growth curve of an organism which is growing on a cellulosic substrate, it is necessary to collect samples from the shake flask at intervals (2-4 hours over 72...
07 July 2013 8,022 10 View
What are the important guidelines for one who wishes to write a review article for publication in a reputable journal for the first time?
04 April 2013 4,659 13 View
In the utilization of lignocellulosic biomass for production of various commodity materials such as bioethanol and others, it is necessary to first hydrolyze the cellulose and hemicellulose...
04 April 2013 3,652 2 View
How do I keep termites alive in a Petri dish for up to a week in the laboratory without them dying? I need to isolate some bacteria from the gut of termites after feeding them on paper for a week,...
04 April 2013 9,461 18 View
I am thinking of producing bioethanol from mixed lignocellulosic substrates (e.g. sugar cane bagasse + corn stover) using microorganisms. Does anyone have any information regarding this idea?
10 October 2012 7,487 5 View
Please can anyone recommend a good book on the study of enzymes (from scratch to purification and analytic techniques) for someone who is new to enzyme studies? Thanks in anticipation.
07 July 2012 1,786 16 View
Do bacteria (esp. lactobacillus) produce cellulase enzyme naturally?
07 July 2012 1,481 3 View
Do lactobacillus strains or other LAB produce cellulase?
07 July 2012 1,376 5 View
