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Questions related from José Avila-Peltroche
Hello all! I have some problems while analyzing my data using two-way ANOVA approach. I have 2 factors (one with 2 levels and the other one with 4 levels) with 3 repetitions in each factor...
02 February 2019 9,211 6 View
Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...
03 March 2018 5,334 4 View
Hello, everybody!I am trying to obtain axenic cultures from filamentous brown algae. In order to test axenic conditions, i m using agar marine sabouraud (for checking fungi) and agar marine (this...
07 July 2017 4,641 3 View
Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....
07 July 2016 7,486 7 View
Hi everybody!I was checking some books and papers related to biostatistics and I found a no clear difference between biological replicates, replicates and repetitions (especially, between the two...
06 June 2016 2,442 13 View
Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...
02 February 2016 4,777 3 View
Hi everybody!I would like to ask your opinion (especially phycologist or anybody who works with plant cell suspension cultures) about this: as we know, Laminariales gametophytes (brown algae)...
02 February 2016 646 0 View
Hi everybody!I'm working with protoplast from U. pinnatifida gametophyte. I was trying several methods only with commercial enzymes (cellulase R-10 and alginate lyase from Sigma) for isolation...
01 January 2016 375 2 View
Hello everybody!I trying to maintain samples of Gelidium elengans for protoplast isolation in sterile Petri dishes containing 100 ml of PES medium at 20°C and medium light intensity (2...
11 November 2015 5,928 8 View
Hello everybody,I was trying to monitoring dead cell in seaweed tissues (in this case of Gelidium). I was trying with Erythrosine and Trypan Blue but do not stain very well dead cells...and even...
11 November 2015 6,232 4 View
I was reading a papers related to two-phase cell suspension culture and the word "interfacial deactivation" appeared several times. What is the definition of this word?Many thanks!
10 October 2015 9,497 2 View
Hi everybody, I want to determinate the asociation between change of paternity (risk factor, nominal) and the presence of pre-eclampsia (nominal) in a large sample of women. My covariate is...
07 July 2015 4,097 7 View
Hi everybody,I was thinking to send an investigation related to Macrocystis pyrifera to the journal Botanica Marina. In its author guidelines there is no mention about publication fee. Does...
05 May 2015 2,129 0 View
Hi everybody. I'm comparing the phenolic content in four different anatomical parts of a seaweed. I made one extraction per part and then I quantified in triplicate. In this case, is it still ok...
04 April 2015 4,830 10 View
I have no normal data or data without homoscedasticity and I'm not very sure if this is suitable for a two way Anova analysis
12 December 2014 1,680 5 View
Hi everybody,I have a big doubt. As a part of my thesis, I am calculating the P and N uptake rate of microalgae using a linear regression, where the slope is the uptake rate. Briefly, in my linear...
12 December 2014 6,074 4 View
