Is it correct to conduct two-way ANOVA when one combination of factors has only zero values?

Hello all! I have some problems while analyzing my data using two-way ANOVA approach. I have 2 factors (one with 2 levels and the other one with 4 levels) with 3 repetitions in each factor...

01 February 2019 9,131 6 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

20 March 2018 3,186 3 View

More chl c than chl a content in brown algae?

Hello everybody! I was extracting pigments (chl a, c and fucoxanthine) from gametophytes cultures of Undaria pinnatifida using DMSO as a solvent. After quantification, my results showed more chl...

02 March 2018 5,256 4 View

What is the most suitable medium for testing axenic condition in macroalgae cultures?

06 July 2017 4,566 3 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

11 July 2016 2,155 6 View

How can I correct baseline shift in an in vivo absorption spectrum?

Hi everybody!I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae....

06 July 2016 7,392 7 View

What is the difference between biological replicates, repetitions and replicates?

Hi everybody!I was checking some books and papers related to biostatistics and I found a no clear difference between biological replicates, replicates and repetitions (especially, between the two...

05 June 2016 2,344 13 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

15 February 2016 4,408 2 View

What kind of mixture design can I use to know the best proportions of enzymes for protoplast isolation?

Hi everybody!In my research, i m isolation protoplast from Undaria pinnatifida gametophyte using cellulase (2%) and alginate lyase (3 U/ml). I was thinking to use a mixture design in order to know...

01 February 2016 4,697 3 View

Does anyone have any advice to improve the protoplast yield from U. pinnatifida gametophyte?

Hi everybody!I'm working with protoplast from U. pinnatifida gametophyte. I was trying several methods only with commercial enzymes (cellulase R-10 and alginate lyase from Sigma) for isolation...

31 December 2015 278 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View