Hello, everybody!
I am trying to obtain axenic cultures from filamentous brown algae. In order to test axenic conditions, i m using agar marine sabouraud (for checking fungi) and agar marine (this is, solidified marine broth; for checking bacteria ) with an incubation time of 2 weeks at 25°C and 28°C, respectively, in the dark. I came with this idea after reading this paper "A new method for removing microflora from macroalgal surfaces" (Klentz et al. 2011). Previous papers in this topic used Zobell 2216 or half-PES with 1% glucose and tryptone as testing mediums under standard conditions (light and temperature according to each macroalgal species).
Should i use Zobell or half-PES medium for texting axenic conditions or the mediums i ve been using are suitable for this?
Thanks in advance
Generally, it is very difficult to get axenic cultures of algae, especially in this approach you are proposing. This is because there may be some interdependence between the algae and the associated microbial community. Once the associated community is removed, interference may occur in the metabolic processes of the seaweed, making it difficult to grow.
Thanks for your answer Adijailton Jose de Souza. I totally agree with that..it is really difficult to get axenic cultures but they are previous reports on that..so i m very optimistic i can get some :)!!
Dear José Avila-Peltroche,
I would like to emphasize that my intention was never to discourage your research. Although difficult, it is not impossible to obtain axenic cultures of algae. Good luck in your search.
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