Hi everybody!

I was making a in vivo absorption spectrum from cultured filamentous brown algae and the spectrum that I have obtained is really similar with previous reports in this kind of algae. However, I have a baseline shift of 0.029 (when I use samples with low OD = 0.020) and 0.073 (when i use samples with higher OD=0.6). I m not sure if there is a way to correct these shifts or if I just simply can present the data like that. As a blank, I m using autoclaved culture medium. 

Also, I have one more question: is it good a 7 nm intervals for making an absorption spectrum? When I made it with 2 nm I obtained too much noise (even with Savitzky-Golay method to smooth the data). With 7 nm intervales I obtain a spectrum really similar to that it is know for brown algae.

Many thanks in advance for your answers

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