I use AKTA Pure for chromatography. I packed CM Sepharose resin in XK50/20 column. my loading flow rate is 28 ml/min which pressure is blow 0.07 MPa. I want to know that the maximum pressure that I can use that doesn't damage the packing, when I load 2CVs of NaOh 1M and 7 CVs of regeneration buffer (0.5 M Sodium Acetate). The CV is 200ml.
Thanks
This product information is for CM Sepharose Fast Flow from CytivaLifeSciences:
Pressure/Flow Specification: 300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm.
This is for CH Sepharose High Performance:
Pressure/Flow Specification Base matrix: 100-200 cm/h, 300 kPa, BioPilot 60/600 column, bed height 30 cm
This is for HiPrep CM FF 16/10 prepacked 20-mol column:
Pressure max. (over the packed bed during operation)1.5 bar [0.15 MPa] (22 psi)
Here is product information from Sigma for CM Sepharose FastFlow, which includes cleaning information, but not pressure limits.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/131/832/ccf100pis-ms.pdf
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