When I started to study about cell line culture I had a doubt that is why we should using the medium containing serum after when cells start coming off from the culture bottle? What material contains in medium containing serum for cell line?
Serum is normally used as a supplement to cell culture media. Because it contains broad spectrum of macro-molecules, carrier proteins for lipoid substances and trace elements, low molecular weight nutrients, and hormones and growth factors and it influence for promoting cell health and cell growth.The most widely used animal serum supplement is fetal bovine serum (FBS).
For more information you can see the reaserch gate web link
https://www.researchgate.net/post/Why_should_we_add_FBS_to_the_serum_free_media
see also this pdf
http://cell-ess.com/biosci/wp-content/uploads/2014/06/FBS_IntroWhitePaper_v8_ae2-SerumApplicability.pdf
best of luck Bhaskara
Dear Bhaskara,
serum is a highly protective solution, containing growth factors, hormones, chelants and detoxifying molecules. Its use supports the viablity of your culture, in fact it is also employed in cell freezing. For instance, when you freeze cells, you can use 50% growth medium, 40% serum and 10% DMSO, while if cells are very important to your work you can employ 90% serum and 10% DMSO.
Good luck with your work,
Davide
Thank you so much..Dear Davide Sir and Omar Faruq sir...Your attachments have good information and really these are most useful to me. Thank you for your good wishes...
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