Omar Faruq

5 Questions 10 Answers 0 Followers

Questions related from Omar Faruq

What is the best protocol to see/measure phagocytosis after knockdown gene in leukemia cells?

Hi everybody, I need a suitable approach/protocol/kit to see siRNA-mediated gene knockdown effect phagocytosis in leukemia cells. I have THP-1 cells, PMA(phorbol 12-myristate-13-acetate), siRNA...

11 August 2021 3,871 0 View

Does anyone have experience phoenix ampho cells transfection using liphofectamin2000 or RNAiMAX reagents?

I have detailed conventional transfection protocol like (i) helper dependent phoenix cell transfection protocol(calcium phosphate protocol) (ii) cationic lipid protocol.

15 March 2017 9,440 0 View

Which Tris is best for annealing buffer recipe ?

I have two types of TRIS (Tris(hydroxymethyl)-aminomethane and Tris (hydroxymethyl)-aminomethane hydrochloride) and I read many recipe for making annealing buffer some people mention TRIS only...

24 February 2017 1,888 3 View

Does anyone have mirZIP(Lentivector-based Anti- MicroRNAs) vector sequence?

I have control plasmid and I am trying to get sequence from this site...

19 February 2017 2,121 0 View

Why are prophages(bacterial DNA and viral DNA containing bacteria) showing resistance to go lytic cycle after treatment with strong doses of UV ?

A bacteriophage attaches itself to the cell wall of a bacterium and injects DNA. Through generalized or specialized transduction, we produced bacteria that carry prophage and remain unharmed and...

01 January 1970 1,767 0 View