If any protocols are available it would be helpful. i have also attached two Fe-SEM images.
It is possible to examine the conventional sections for light microscopy or ultrathin sections for TEM by SEM after coating (5-10 nm gold, etc). In BSD mode you will have very informative images.
You can examine the sections without coating in VP mode, but it does not have any advantages.
Dear Debasis,
Regarding this it is better to consult the techician of the SEM machine as mostly for gold coating of samples, they prefer dry sample.
In your case the cells may have to be transferred from dried smear to the SEM mount.
All the best,
Prakash
Prakash i am asking for the method to fix the cells for better viewing the cells in SEM. I did the gold coating but the morphology of the cells were distorted when i saw the slides in Fe-SEM.
Debasis ....
before anyone will perhaps reply in a polite and serious manner you certainly - with regard to the method (s)/ steps of processing you made until observing in the SEM first (ee your Re #3) - should put your cards on the table...please detailed information then you'll get for sure answers (and: usually one will know how to fix cells for SEM -observation ...)
{[NB: I will not be able to respond earlier than 30th of Sept due to ann.leave/vacation.]}
The method which i followed is: Fix the cells with 3% glutaraldehyde followed by successive washing with 30%, 50%, 70%, 80%, 90% and 95% alcohol. followed by overnight drying at room temperature. But when i am viewing them in SEM the next day, the cells are distorted. I am not able to view the correct morphology of the cells.
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