I am using premade gels (4-12%) and using Running Buffer Tris Glycine Buffer and Licor SDS loading buffer
soil fungal diversity of hevea brasiliensis
14 June 2024 9,389 0 View
I have human brain tissue samples that I lyse with a nuclear extraction buffer and then the pellet I solubilized in 8M urea. I run samples in Tris-Glycine buffer in premade 4-12% gels. Ran the gel...
04 March 2024 9,722 0 View
I have a fresh water red algae. Which media will be suitable to grow it ?
26 February 2024 8,391 1 View
I am interested in learning about environmental science, climate change, and sustainable development concepts in children's and teachers minds and would like to do some research and review on the...
21 February 2024 2,113 3 View
Need some quick publishing reputed name of journal for education, Science education field
07 February 2024 4,799 1 View
NiOCNF added with PVDF matrix , why its showing the Ferrimagnetic behaviour
15 January 2024 8,433 0 View
Hi, I'm using the VMD pathways plugin for the first time. I'm analyzing a trajectory, these are few issues I'm facing while doing it. I'll be grateful for any insight. 1. When I'm giving the...
11 December 2023 6,636 0 View
If we use undoped materials and record their PL intensity, the intensity of the sample rises or decreases after doping. We need a low rate of recombination with a higher charge carrier...
04 December 2023 2,368 1 View
I have run these westerns a billion times and something or the other doesn’t work out. For eg, my gel ran straight and fine but when I transfer the gel to my membrane, half of the membrane shows...
27 November 2023 1,494 0 View
I want to detect the methylation status for GBM patient samples. I am confused about getting pyrosequencing or MSP. Also, all the MSP kits I found are costly. Please suggest an efficient and...
30 October 2023 8,432 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View