Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Can we measure porosity of a composite by DSC (heat flow measurements)?

In the DSC conducted for A and B (image provided). B is a derivative of A. Can I conclude that the porosity of composite B is more than A, Since the heat flow is decreasing with increase in...

26 December 2020 9,104 3 View

Can I carry out LC-MS for AuNPs?

Will it be helpful to know the nature of stabilizers used in synthesis of curcumin (if it has polymerized or not). Any specific measures to be followed to carry out these kind of experiments?

15 December 2020 2,283 4 View

For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

Why I am not getting UV absorbance peak of AuNPs?

I synthesized Au deposited on graphite electrochemically by using chronopotentiometry. How much ever I tried I couldn't get UV absorbance peak of AuNPs in any number of solutions I tried? But when...

20 October 2020 2,040 5 View

Can i use surfactant C-TAB in extraction and detection of the aminaphthalene sulfonates by HPLC technique?

In the detection of Aminonaphthalene sulfonates the quaternary ammonium salts like Tetrabutyl ammonium bromide ,Triethyl ammonium acetates are used so the C-TAB being an quarternary ammonium...

21 September 2020 9,636 1 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

Which motion analysis software can give me this functionality to create some sort of kinematic model?

25 October 2019 2,556 6 View

Automation in ansys with load location as parameter?

What is the best way (in a way that ansys runs each job sequentially without manual command) to solve several problems (say 1000+) with exact same geometry and other solution parameters but...

07 October 2019 2,073 3 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Which period is considered as study period in research?

Is that starting from proposal development to result writeup or just from data collection to result writeup and reporting (publishing)?

28 February 2021 910 2 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View