Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

Can we measure porosity of a composite by DSC (heat flow measurements)?

In the DSC conducted for A and B (image provided). B is a derivative of A. Can I conclude that the porosity of composite B is more than A, Since the heat flow is decreasing with increase in...

26 December 2020 9,104 3 View

Can I carry out LC-MS for AuNPs?

Will it be helpful to know the nature of stabilizers used in synthesis of curcumin (if it has polymerized or not). Any specific measures to be followed to carry out these kind of experiments?

15 December 2020 2,283 4 View

For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

Why I am not getting UV absorbance peak of AuNPs?

I synthesized Au deposited on graphite electrochemically by using chronopotentiometry. How much ever I tried I couldn't get UV absorbance peak of AuNPs in any number of solutions I tried? But when...

20 October 2020 2,040 5 View

Can i use surfactant C-TAB in extraction and detection of the aminaphthalene sulfonates by HPLC technique?

In the detection of Aminonaphthalene sulfonates the quaternary ammonium salts like Tetrabutyl ammonium bromide ,Triethyl ammonium acetates are used so the C-TAB being an quarternary ammonium...

21 September 2020 9,636 1 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

Which motion analysis software can give me this functionality to create some sort of kinematic model?

25 October 2019 2,556 6 View

Automation in ansys with load location as parameter?

What is the best way (in a way that ansys runs each job sequentially without manual command) to solve several problems (say 1000+) with exact same geometry and other solution parameters but...

07 October 2019 2,073 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Can I simply dilute 25% NH3 solution to 10% solution by pouring water in tank and circulating with pump?

Is there any heat of dilution which I need to take care by putting some circulation cooler? Will there be any significant ammonia emission in the process?

09 February 2021 5,943 2 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

What contributions could be done in the medical emergency field based on voice recognition and NLP?

Hello, My research work focuses on the use of NLP and voice recognition for the medical emergency assistance. So I would appreciate it if you suggest me some contributions that could be done in...

27 January 2021 2,899 3 View

What are the IT and Artificial intelligence contributions to assisting medical emergency cases?

Hello, I am seeking recommendations from the Research Gate community regarding studies about the IT and Artificial intelligence solutions to assisting medical emergency cases. What are the...

23 January 2021 2,667 21 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How has Artificial Intelligence impacted Healthcare?

The evolution of Artificial Intelligence is influencing healthcare. With the production of applications such as AI-based biomedical applications, and medical AI systems, etc. Without a doubt, AI...

20 January 2021 3,971 11 View

The First Survey Addressing Patients with BMIs over 50?

Dear Bariatric Surgeons, The importance of obesity management in patients with BMIs over 50 has not been completely addressed and it should be considered separately from patients with morbid...

20 January 2021 2,748 1 View