From where can I get cisplatin resistant breast cancer cell line?

I do research in Kolkata, India?

09 October 2019 9,266 3 View

From where can I get radioressistant kidney cancer and breast cancer cell line?

The cell lines should be radiation resistant

09 October 2019 7,702 1 View

What conc of peptide should be taken to check immunogenicity in in vitro T cell priming experiment?On what criterias does the conc of peptides depend?

I am using 4 mM for in vivo experiment. What should be the concentration for in vitro T cell priming experiment

03 April 2019 616 1 View

What are the factors on which the concentration of endocytic inhibitors (eg Cyto D, EIPA,NaN3 etc)to be used in a study for any transporter depend?

Does it depend on the cell line

10 November 2018 1,551 0 View

For determining IC50 with MTT what time point should I use?

Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231

09 October 2018 3,133 3 View

Has anybody used Erk1/2 antibody from santa cruz(c9)CATALOG:sc-514302 in MDA-MB-231? How is the expression of this Ab in non treated MDA-MB-231?

Is this Ab working well? How is the expression level?

04 May 2018 5,277 3 View

I culture my cells in RPMI-1640, will it be harmful for the cell if i use opti-MEM for some some time for certain experiments?

In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...

04 May 2018 1,126 4 View

What problem can arise in Western and Immunofluorescence if my primary antibody consists of only kappa light chains and not the whole antibody?

What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?

01 February 2018 6,243 1 View

Can primary antibodies used for western can also be used for immunocytochemistry?CST says they haven't tried their Ab for fluorescent studies?

If all primary Ab used for western can't be used for immunocytochemistry what is the reason behind it? The primary antibodies sold by Abcam says that immunocytochemistry can be done with it but...

31 December 2017 3,895 5 View

I want to check upregulation of receptors and other proteins in a tissue lysate. What method should I follow?

Please refer papers with detailed protocol that can be understood for biology students

11 December 2017 1,382 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View