The cell lines should be radiation resistant
I don't know of any cell lines that are, but if you have breast cancer or kidney cancer cell lines (available from your local supplier) you can make them radio-resistant:
Article Development and characterisation of acquired radioresistant ...
I do research in Kolkata, India?
09 October 2019 9,368 3 View
I am using 4 mM for in vivo experiment. What should be the concentration for in vitro T cell priming experiment
03 April 2019 724 1 View
Does it depend on the cell line
10 November 2018 1,634 0 View
Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231
09 October 2018 3,226 3 View
Is this Ab working well? How is the expression level?
04 May 2018 5,365 3 View
In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...
04 May 2018 1,220 4 View
What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?
01 February 2018 6,344 1 View
If all primary Ab used for western can't be used for immunocytochemistry what is the reason behind it? The primary antibodies sold by Abcam says that immunocytochemistry can be done with it but...
31 December 2017 3,984 5 View
Please refer papers with detailed protocol that can be understood for biology students
11 December 2017 1,475 3 View
Among the three which would give the best result with highest yield and why. Considering post translational modification and glycosylation is necessary for our membrane protein.
11 December 2017 3,795 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View