What conc of peptide should be taken to check immunogenicity in in vitro T cell priming experiment?On what criterias does the conc of peptides depend?

04 April 2019 1 724 Report

I am using 4 mM for in vivo experiment. What should be the concentration for in vitro T cell priming experiment

Hi,

4mM in which volume? I used in the past (see the excellent paper of 1995 attached ;-)) 50microg to induce in vivo a CD4 T cell response

for in vitro priming of CD4 T Cells, 10 microg/ml is used. that is a lot and probably in excess (paper attatched). I guess for CD8 T cells lower concentration could be used

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