From where can I get cisplatin resistant breast cancer cell line?

I do research in Kolkata, India?

09 October 2019 9,266 3 View

From where can I get radioressistant kidney cancer and breast cancer cell line?

The cell lines should be radiation resistant

09 October 2019 7,702 1 View

What are the factors on which the concentration of endocytic inhibitors (eg Cyto D, EIPA,NaN3 etc)to be used in a study for any transporter depend?

Does it depend on the cell line

10 November 2018 1,551 0 View

For determining IC50 with MTT what time point should I use?

Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231

09 October 2018 3,133 3 View

Has anybody used Erk1/2 antibody from santa cruz(c9)CATALOG:sc-514302 in MDA-MB-231? How is the expression of this Ab in non treated MDA-MB-231?

Is this Ab working well? How is the expression level?

04 May 2018 5,277 3 View

I culture my cells in RPMI-1640, will it be harmful for the cell if i use opti-MEM for some some time for certain experiments?

In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...

04 May 2018 1,126 4 View

What problem can arise in Western and Immunofluorescence if my primary antibody consists of only kappa light chains and not the whole antibody?

What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?

01 February 2018 6,243 1 View

Can primary antibodies used for western can also be used for immunocytochemistry?CST says they haven't tried their Ab for fluorescent studies?

If all primary Ab used for western can't be used for immunocytochemistry what is the reason behind it? The primary antibodies sold by Abcam says that immunocytochemistry can be done with it but...

31 December 2017 3,895 5 View

I want to check upregulation of receptors and other proteins in a tissue lysate. What method should I follow?

Please refer papers with detailed protocol that can be understood for biology students

11 December 2017 1,382 3 View

I want to prepare human membrane proteins. Expressing the protein in yeast, insect cell or in cell free extract which would give me the best result?

Among the three which would give the best result with highest yield and why. Considering post translational modification and glycosylation is necessary for our membrane protein.

11 December 2017 3,640 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

03 March 2021 5,360 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View