Argha Mario Mallick

15 Questions 4 Answers 0 Followers

Questions related from Argha Mario Mallick

From where can I get radioressistant kidney cancer and breast cancer cell line?

The cell lines should be radiation resistant

10 October 2019 7,766 1 View

From where can I get cisplatin resistant breast cancer cell line?

I do research in Kolkata, India?

10 October 2019 9,342 3 View

What conc of peptide should be taken to check immunogenicity in in vitro T cell priming experiment?On what criterias does the conc of peptides depend?

I am using 4 mM for in vivo experiment. What should be the concentration for in vitro T cell priming experiment

04 April 2019 692 1 View

What are the factors on which the concentration of endocytic inhibitors (eg Cyto D, EIPA,NaN3 etc)to be used in a study for any transporter depend?

Does it depend on the cell line

11 November 2018 1,600 0 View

For determining IC50 with MTT what time point should I use?

Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231

10 October 2018 3,197 3 View

I culture my cells in RPMI-1640, will it be harmful for the cell if i use opti-MEM for some some time for certain experiments?

In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...

05 May 2018 1,187 4 View

Has anybody used Erk1/2 antibody from santa cruz(c9)CATALOG:sc-514302 in MDA-MB-231? How is the expression of this Ab in non treated MDA-MB-231?

Is this Ab working well? How is the expression level?

05 May 2018 5,335 3 View

What problem can arise in Western and Immunofluorescence if my primary antibody consists of only kappa light chains and not the whole antibody?

What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?

02 February 2018 6,307 1 View

Can primary antibodies used for western can also be used for immunocytochemistry?CST says they haven't tried their Ab for fluorescent studies?

If all primary Ab used for western can't be used for immunocytochemistry what is the reason behind it? The primary antibodies sold by Abcam says that immunocytochemistry can be done with it but...

01 January 2018 3,960 5 View

I want to check upregulation of receptors and other proteins in a tissue lysate. What method should I follow?

Please refer papers with detailed protocol that can be understood for biology students

12 December 2017 1,443 3 View

I want to prepare human membrane proteins. Expressing the protein in yeast, insect cell or in cell free extract which would give me the best result?

Among the three which would give the best result with highest yield and why. Considering post translational modification and glycosylation is necessary for our membrane protein.

12 December 2017 3,759 1 View

Where can I get unmodified siRNA (not modified to be stable; native as present in human body) to perform serum stability assay?

I'm from India. Can anyone pls give me the information and catalog number of this products from reputed companies like Dharmacon, Cell signalling etc

10 October 2017 890 2 View

What is the detailed protocol to make long peptides using biotechnological approach?

Please provide the references. The peptides would be used for therapeutic purposes for humans

10 October 2017 7,792 3 View

What is the percentage of knockdown if I use HiPerFect transfecting agent on MDA MB231 cell line?

mainly for ERK1 silencing

08 August 2017 7,914 2 View

What is the protocol for culturing cell line SK-RC-45 & SK-RC26b? Are these cells from ccRCC or papillary RCC histology?

Any other detailed information about these cell lines would be highly useful to me.

08 August 2017 7,461 2 View