Hi
I usually work with primary keratinocytes but am looking at performing some work with HaCaT cells - can I ask what is the optimal passage to use these up until? I currently am growing some at passage 42
Kind regards
Maheshkumar P Patil
Hello,
I am not sure about how long u can passage cells, but in my opinion you can continue until cells have no changes in their morphology.
Generally, cell culture passage preferred to less passage, because cells facing stress and nutritional starvation during growth and sub-culturing that makes some morphological changes. Better-way, you can prepare stock of cells and try to use early passage cells for your experiment.
Good day.
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