How to define the profile so to receive a Nobel price or be part of Nobel Assembly? Who controls Nobel Assembly?

08 September 2016 1,058 4 View

What is autoimmunity? What are the consequences for the reserved system and its components? Apoptosis and autoimmunity are two antipodal points?

01 February 2016 1,259 3 View

Is the biochemical property of a protein representative for its biological function?

The current progress in biomedical science works on the basis of the identification of a gene, a protein, a biochemical function and a biological activity followed by a possible drug...

11 December 2015 10,050 4 View

No title

11 December 2015 3,528 3 View

Why the Topological properties of a Research center or a Building-Administration is dominant in the creative activities of the Scientists working in?

A scientist has a scientific record A established during his career. In day 1 he joins the University of XX for a tenure tract position or he/she joined a scientific organization (CNRS) or the...

09 October 2015 2,303 0 View

Is corruption the major problem of scientific integrity in public and academic research centers?

I am providing below a recent comment appeared in Nature providing a link between 3D-organization and corruption in public research centers as a basis of reflection and discussion. There are many...

09 October 2015 2,061 3 View

Is the FEAR the basis of control of our lives and our hopes by the political and economic establishment?

09 October 2015 4,873 6 View

How can I determine if a phenotype effect is Genetic, Epigenetic or ChoroEpigenetic?

02 March 2015 865 6 View

Is human health a business or a human right? What is your opinion?

01 January 1970 2,531 46 View

No title

By definition any system including the brain is part of the environment and it should reflect basic properties of 'matter in motion as a whole'. The brain reflects part of the environmental system...

01 January 1970 9,211 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View