Ritika Vishnoi

7 Questions 1 Answers 0 Followers

Questions related from Ritika Vishnoi

CRISPR Cas9 based single nucleotide change in rice?

Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9

02 April 2023 5,198 0 View

During conventional stickey end cloning, dies the ratio of vector to insert depends on the size of the vector and insert?

I am trying to clone h 533 bp fragment in a 21000bp vector. What should be appropriate ratio of vector to insert to use for successful cloning.

01 January 2023 6,707 3 View

Which polymerase is good for amplifying 4.5kb fragment from a plasmid?

29 December 2022 9,048 5 View

What is the maximum size of amplicon amplified by pfu polymerase?

I want to amplify a 4.5kb fragment from a plasmid. Can pfu polymerase from genebio is suitable for the job?

29 December 2022 1,346 3 View

Is it necessary to use T4 polynucleotide kinase during annealing oligos for guide RNA cloning?

I am having difficulties in ligating guide RNA in a vector, digestion happens but ligation doesn't. I am not using T4 polynucleotide kinase during oligo annealing ( Zhang's lab protocol). So, I...

25 September 2022 7,229 0 View

Golden Gate cloning using Bbs1 not working?

I am trying to assemble 5 different construct in a single destination vector but my digestion ligation reaction is not working. I am getting all blue colonies except one or two white colonies. By...

11 July 2022 339 0 View

Does the two guide RNA for CRISPR can be such that one is on sense and other on antisense strand?

While designing theguide RNA for CRISPR we prefer to use two guide RNA for greater knockout efficiency, So can we design the two guide RNA one from sense and other from antisense strand?

07 March 2022 7,798 2 View