Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9
This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...
01 November 2020 5,660 1 View
In respect to a Neural Network.
30 June 2019 9,728 3 View
I need to implement a feature selection method for my dataset and I am asked to use feature selection based on covariance. Therefore I want to understand whether the feature correlation method...
28 May 2019 2,547 8 View
Most of the study have used confusion metrics and performance measures like accuracy, recall, precision and f-score for presenting the results and comparing the machine learning methods. What is...
24 February 2019 5,329 3 View
why we need to bring back the image from frequency domain to spatial domain. please advice and share some related materials.
05 August 2018 1,934 4 View
To test for proliferative cells, I stained my tissue with Ki67- primary overnight and secondary 30min. This was followed by ABC 30min and DAB (1mL buffer + 1drop chromogen) for 30min. I...
27 July 2018 469 2 View
Hello Researchers, can anyone tell me how to generate CNN model for any data (expect image, because hat i have gone through till now, it has been done on the image data only), what should be in...
16 April 2018 4,005 3 View
13 March 2015 1,438 3 View
I would like to mention that i am using terminelia arjuna aq. extract and want to do HPLC using arjunolic acid as standard.
03 February 2015 3,615 3 View
How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?
26 December 2014 5,204 2 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?
08 February 2021 2,343 6 View
I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...
08 February 2021 2,032 2 View
can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...
07 February 2021 470 2 View
Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...
05 February 2021 5,842 3 View
03 February 2021 221 2 View
I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...
02 February 2021 8,083 1 View
Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...
31 January 2021 2,516 1 View
The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?
30 January 2021 1,420 3 View