Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9
hi, can someone help with possible ways of doing content analysis from annual reports of Indian companies. I am looking at risk disclosure practices of companies and how can i create a risk...
13 July 2023 1,075 3 View
I am trying to clone h 533 bp fragment in a 21000bp vector. What should be appropriate ratio of vector to insert to use for successful cloning.
31 December 2022 6,736 3 View
Which polymerase is good for amplifying 4.5kb fragment from a plasmid?
28 December 2022 9,065 5 View
I want to amplify a 4.5kb fragment from a plasmid. Can pfu polymerase from genebio is suitable for the job?
28 December 2022 1,412 3 View
I have DNA isolated from Leishmania parasites using a Qiagen kit. Please guide me how to lyophilize this DNA sample.
08 November 2022 292 2 View
I am having difficulties in ligating guide RNA in a vector, digestion happens but ligation doesn't. I am not using T4 polynucleotide kinase during oligo annealing ( Zhang's lab protocol). So, I...
24 September 2022 7,248 0 View
I am looking for a vector which allows translational fusion of gene of interest with GFP for wheat. Does anyone know about any such vector.
13 September 2022 6,204 0 View
I am trying to assemble 5 different construct in a single destination vector but my digestion ligation reaction is not working. I am getting all blue colonies except one or two white colonies. By...
10 July 2022 359 0 View
I am looking for a good formula to compute % canopy cover for the trees of a plot. I have the dbh for all the trees, calcualted crown projection area (using the crown radius of the species),...
10 May 2022 1,939 0 View
While designing theguide RNA for CRISPR we prefer to use two guide RNA for greater knockout efficiency, So can we design the two guide RNA one from sense and other from antisense strand?
06 March 2022 7,809 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
I work on producing organic fertilizer from rice husk. how can I reduce the bio-conversion time ?less than two months
20 July 2024 6,049 3 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View