How to lyophilize isolated DNA sample in a simple way?

More Ritika Sengupta's questions See All

Is it justified to compare the free energy change of two reactions and conclude one is more favourable than the other?

Suppose we consider the binding of a ligand L to a metal M, M+L=ML and M+2L=ML2 . We compute the Gibbs free energy change for two reaction using DFT. But can we compare this two free energies to...

12 January 2021 5,893 4 View

Does Tea Garden proves to be an alternative to forest landscape?

Is there possible that the Tea Gardens proves to be an alternative habitat to forest dwelling organisms? If yes, what pressure do they face in relation to anthropogenic pressure?

03 January 2021 429 13 View

How to run solver in Nektar++?

Hi! I am introducing myself very new to Nektar++ (https://www.nektar.info/) opensource code. I installed the code according to the user guide manual. The installing process shows success. --...

02 January 2021 8,977 3 View

Relating SOMO-LUMO gap and stability for a Open shell system (Unrestricted Calculation)?

It is well known that for a closed shell (all electrons are paired up) molecule, the HOMO-LUMO gap is related to its stability. However, I often see that the same argument is used for open-shell...

01 December 2020 6,771 4 View

How can we block ROS production in-vivo in mice?

I want to block ROS production in mice and, therefore, looking for probable inhibitory compounds/protocols. I would appreciate it if anybody shares his/her knowledge of this.

25 August 2020 7,265 4 View

Where to get dark networking data?

Hi, I want to study the dark network structure (social network for criminals). I want to study the network connections, important factors, significant leaders of the network. I already found one...

23 August 2020 4,239 3 View

How do I generate the residuals in STATA after a CCE-GMM estimation?

I used the following codes to use the CC-GMM estimator developed by Neal (2015). I have treated the immigration variable (LIMM) as the endogenous variable. I attempted to instrument the endogenous...

16 August 2020 9,999 4 View

Should I use the absolute value of a correlation coefficient?

I have calculated the Pearson correlation coefficient for two sets of values using excel. I got the value in the negative number. I know that the idea of getting a negative correlation means that...

24 July 2020 6,317 13 View

How to create lncRNA miRNA network?

Hi, I am trying to make a network between DElncRNAs and DEmiRNAs. Most of the DElncRNAs having NONHSAT, NONHSAG IDs. Is there any database or online tool which can map these IDs to miRNAs? I have...

10 April 2020 5,168 1 View

Puzzle1 Shake-hand Solution?

Hi All, As most of us are home quarantined and may not have enough chance to pump our brain muscles, herein I am introducing a brainstorming session, Puzzle Series. Just a bit refreshment within...

31 March 2020 7,111 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View