Can i apply bell method to equilize the histogram in ImageJ, please let me know the way if that can be done ?

This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...

01 November 2020 5,660 1 View

Hello dear all. How will Epoch, Batch Size and Iteration be differenciating to each other and what is the correlation among them?

In respect to a Neural Network.

30 June 2019 9,728 3 View

How is correlation different from covariance ?

I need to implement a feature selection method for my dataset and I am asked to use feature selection based on covariance. Therefore I want to understand whether the feature correlation method...

28 May 2019 2,547 8 View

What is the significance of performance metrics used for evaluating the machine learning models?

Most of the study have used confusion metrics and performance measures like accuracy, recall, precision and f-score for presenting the results and comparing the machine learning methods. What is...

24 February 2019 5,329 3 View

Why we used inverse Fourier transform to get back the image?

why we need to bring back the image from frequency domain to spatial domain. please advice and share some related materials.

05 August 2018 1,934 4 View

Can counterstaining override DAB in Ki67?

To test for proliferative cells, I stained my tissue with Ki67- primary overnight and secondary 30min. This was followed by ABC 30min and DAB (1mL buffer + 1drop chromogen) for 30min. I...

27 July 2018 469 2 View

How to construct CNN Model for given description?

Hello Researchers, can anyone tell me how to generate CNN model for any data (expect image, because hat i have gone through till now, it has been done on the image data only), what should be in...

16 April 2018 4,005 3 View

How does adding buffer in path fixes the set up violation?

13 March 2015 1,438 3 View

Can someone suggest a protocol for HPLC with aqueous plant extract?

I would like to mention that i am using terminelia arjuna aq. extract and want to do HPLC using arjunolic acid as standard.

03 February 2015 3,615 3 View

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

26 December 2014 5,204 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View