I am looking for a vector which allows translational fusion of gene of interest with GFP for wheat. Does anyone know about any such vector.
hi, can someone help with possible ways of doing content analysis from annual reports of Indian companies. I am looking at risk disclosure practices of companies and how can i create a risk...
13 July 2023 1,075 3 View
Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9
01 April 2023 5,222 0 View
I am trying to clone h 533 bp fragment in a 21000bp vector. What should be appropriate ratio of vector to insert to use for successful cloning.
31 December 2022 6,736 3 View
Which polymerase is good for amplifying 4.5kb fragment from a plasmid?
28 December 2022 9,065 5 View
I want to amplify a 4.5kb fragment from a plasmid. Can pfu polymerase from genebio is suitable for the job?
28 December 2022 1,412 3 View
I have DNA isolated from Leishmania parasites using a Qiagen kit. Please guide me how to lyophilize this DNA sample.
08 November 2022 292 2 View
I am having difficulties in ligating guide RNA in a vector, digestion happens but ligation doesn't. I am not using T4 polynucleotide kinase during oligo annealing ( Zhang's lab protocol). So, I...
24 September 2022 7,248 0 View
I am trying to assemble 5 different construct in a single destination vector but my digestion ligation reaction is not working. I am getting all blue colonies except one or two white colonies. By...
10 July 2022 359 0 View
I am looking for a good formula to compute % canopy cover for the trees of a plot. I have the dbh for all the trees, calcualted crown projection area (using the crown radius of the species),...
10 May 2022 1,939 0 View
While designing theguide RNA for CRISPR we prefer to use two guide RNA for greater knockout efficiency, So can we design the two guide RNA one from sense and other from antisense strand?
06 March 2022 7,809 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?
28 July 2024 4,886 2 View