Can i apply bell method to equilize the histogram in ImageJ, please let me know the way if that can be done ?

This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...

01 November 2020 5,660 1 View

Hello dear all. How will Epoch, Batch Size and Iteration be differenciating to each other and what is the correlation among them?

In respect to a Neural Network.

30 June 2019 9,728 3 View

How is correlation different from covariance ?

I need to implement a feature selection method for my dataset and I am asked to use feature selection based on covariance. Therefore I want to understand whether the feature correlation method...

28 May 2019 2,547 8 View

What is the significance of performance metrics used for evaluating the machine learning models?

Most of the study have used confusion metrics and performance measures like accuracy, recall, precision and f-score for presenting the results and comparing the machine learning methods. What is...

24 February 2019 5,329 3 View

Why we used inverse Fourier transform to get back the image?

why we need to bring back the image from frequency domain to spatial domain. please advice and share some related materials.

05 August 2018 1,934 4 View

Can counterstaining override DAB in Ki67?

To test for proliferative cells, I stained my tissue with Ki67- primary overnight and secondary 30min. This was followed by ABC 30min and DAB (1mL buffer + 1drop chromogen) for 30min. I...

27 July 2018 469 2 View

How to construct CNN Model for given description?

Hello Researchers, can anyone tell me how to generate CNN model for any data (expect image, because hat i have gone through till now, it has been done on the image data only), what should be in...

16 April 2018 4,005 3 View

How does adding buffer in path fixes the set up violation?

13 March 2015 1,438 3 View

Can someone suggest a protocol for HPLC with aqueous plant extract?

I would like to mention that i am using terminelia arjuna aq. extract and want to do HPLC using arjunolic acid as standard.

03 February 2015 3,615 3 View

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

26 December 2014 5,204 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View