Can i apply bell method to equilize the histogram in ImageJ, please let me know the way if that can be done ?

This question is regarding the use of imageJ, I want to apply the same effect to my image that can be done by the command "ret = IpHstEqualize(EQ_BELL)" in image pro premier. I have imageJ only...

01 November 2020 5,660 1 View

Hello dear all. How will Epoch, Batch Size and Iteration be differenciating to each other and what is the correlation among them?

In respect to a Neural Network.

30 June 2019 9,728 3 View

How is correlation different from covariance ?

I need to implement a feature selection method for my dataset and I am asked to use feature selection based on covariance. Therefore I want to understand whether the feature correlation method...

28 May 2019 2,547 8 View

What is the significance of performance metrics used for evaluating the machine learning models?

Most of the study have used confusion metrics and performance measures like accuracy, recall, precision and f-score for presenting the results and comparing the machine learning methods. What is...

24 February 2019 5,329 3 View

Why we used inverse Fourier transform to get back the image?

why we need to bring back the image from frequency domain to spatial domain. please advice and share some related materials.

05 August 2018 1,934 4 View

Can counterstaining override DAB in Ki67?

To test for proliferative cells, I stained my tissue with Ki67- primary overnight and secondary 30min. This was followed by ABC 30min and DAB (1mL buffer + 1drop chromogen) for 30min. I...

27 July 2018 469 2 View

How to construct CNN Model for given description?

Hello Researchers, can anyone tell me how to generate CNN model for any data (expect image, because hat i have gone through till now, it has been done on the image data only), what should be in...

16 April 2018 4,005 3 View

How does adding buffer in path fixes the set up violation?

13 March 2015 1,438 3 View

Can someone suggest a protocol for HPLC with aqueous plant extract?

I would like to mention that i am using terminelia arjuna aq. extract and want to do HPLC using arjunolic acid as standard.

03 February 2015 3,615 3 View

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

How can I dock a protein with its cofactor using CLC Drug Discovery workbench so that I can find all the interactions individually in binding sites?

26 December 2014 5,204 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View