I have had a request for 15 µm sections from very precious TMAs. I don't think this will work, because thick sections usually roll, the individual cores usually fold if the section is too thick. I usually section them at 3-5 µm.
Does anyone have any experience of cutting TMAs thicker?
I have never attempted this, but you could try asking Rudi Billeter (Nottingham) - it's possible he might know. Email: [email protected]
My first question is why would somebody need such thick sections? Perhaps you can persuade them to take a more rational approach. I have cut many TMAs and I agree that the sections would roll and be difficult to float. There would be very likely tissue loss when the slides are stained.
Thanks John. I was not convinced that we could justify thicker sections, and we have agreed that they will receive 5 µm sections as usual.
- Badges
- Science topic