Dear All,
We have ordered THP-1 cells from NCCS, Pune and we recieved the THP-1 cells after a week (from the day it was dispatched to the day it was received it was 7 days) in a T-flask filled with the Transport medium. All the cells were attached when it is supposed to be a suspension culture ... What can be the reason for that .... and how many days the cells can survive the transport?
Is the Transport medium different from normal RPMI 1640 medium?
Thank you.
Cells can survive for more than 2 weeks. The PetakaG3 system for example creates a hypoxic condition increasing survival of adherent cells up to 3 weeks: http://celartia.com/images/Files_download/Shipping%20cell%20cultures%20without%20cryopreservation%20in%20petaka%20g3.pdf
THP-1 cells become adherent when they differentiate. You probably have a situation where your cells survived, but they have been subject to possibly permanent epigenetic changes.
Cells can survive for more than 2 weeks. The PetakaG3 system for example creates a hypoxic condition increasing survival of adherent cells up to 3 weeks: http://celartia.com/images/Files_download/Shipping%20cell%20cultures%20without%20cryopreservation%20in%20petaka%20g3.pdf
THP-1 cells become adherent when they differentiate. You probably have a situation where your cells survived, but they have been subject to possibly permanent epigenetic changes.
Trasport medium is containing less amount of FBS 2-5% in the regular (RPMI or DMEM) medium. Cy at theell survival depends on the seeding density at the time of dispatch of the cell line. If less number of cells are seeded in the beginnig then it will gives viability and depends on your luck.
all the best
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