Dear All,
Please help out ... We had ordered for HIG-82 Cell line (Synoviocytes of Rabbit) and it is delivered today. And the cells are confluent and ready to be split in to sub cultures. But, the Ham's F12K medium prescribed for the cells is still in transit. And we have DMEM, RPMI and MEM(E) media available in our lab.
In worst case scenario, can any one of these media can be used for the HIG-82 cell lines before the Ham's F12 K arrives.
Please help me in this regard...
Its is a continuous cell line from soft tissue lining the knee joints of rabbits. HIG-82 cells can be activated by a number of stimuli, including phorbol myristate acetate (PMA), interleukin-1 (IL-1), and the endocytosis of latex beads. Activated cells secrete collagenase, gelatinase, caseinase (stromelysin), and prostaglandin E2 (PGE2) into their culture medium. prostaglandin E 2 (PGE 2) are added into their culture medium. You can try RPMI-140 with 10% serum or Dulbecco's modified Eagle's medium (DMEM)/Nutrient mixture and replace Ham's F12K medium.
Sir!
Thanks a lot!
But, temporarily if i am using dmem medium and once the Ham's F12K medium arrives ... if I shift the cells to that ... will the cells adjust and grow well?
Dear Saikrishna
As you have got a confluent culture, I will advise to use all three media with 10% FBS in three different T-25 flasks and you can see which of them works best.
Answer to your second question that wheather you can shift the cells if HAM's arrive then the best way to do that is once you acheive a confluence, make a stock in -80 or liq. Nitrogen and then revive the same in HAM's media. If you want to change the media in a running culture then you have to make your cells adapt for that. In that case u can use different volumes like 75% ongoing media+ 25% HAM's media, then 50-50 ration in next turn and finally at fourth round you can arrive at HAM's media with good cell health.. Hope it will help
Dear All,
Thanks a lot for your valuable suggestions...as suggested by you...
Actually I tried DMEM and RPMI... The cells seem to be growing fine in DMEM medium but RPMI did not work out!
I got the HAM's F12K medium and now I shifted them to the new medium. They are growing well.
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