Can anyone suggest a protocol to do a phagocytosis assay using RAW 264.7 cells?

Hi everyone, I would like to do a phagocytosis assay with my selected compound using RAW 264.7 cells. I want to know in what cell culture plate I should be doing this experiment - 6 well, 24 well...

08 September 2015 911 8 View

Is there anyone who is working on chondrocyte cell lines in India?

Dear All, I am currently working on primary chondrocyte cells which are limited with the no. of passages they can be grown. I would like to know if anybody is working in India on chondrocyte cell...

02 March 2015 8,690 4 View

For doing a catalase enzyme assay, what should be the protocol I should follow to lyse the chondrocytes (primary cells)?

01 February 2015 3,137 4 View

Which medium (DMEM, RPMI or MEM(E)) to use with the HIG-82 cell line can replace temporarily the prescribed Ham's F12K medium ?

Dear All, Please help out ... We had ordered for HIG-82 Cell line (Synoviocytes of Rabbit) and it is delivered today. And the cells are confluent and ready to be split in to sub cultures. But, the...

11 December 2014 3,157 4 View

What is the maximum no. of days that cells can survive in transport medium once ordered from a cell repository?

Dear All, We have ordered THP-1 cells from NCCS, Pune and we recieved the THP-1 cells after a week (from the day it was dispatched to the day it was received it was 7 days) in a T-flask filled...

10 November 2014 2,138 3 View

Can you please suggest me the best cell line for Rheumatoid Arthritis?

08 September 2014 5,177 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View