I am interested in doing anti-oxidant studies for rheumatoid arthritis.
There are many cell types involved in RA. The most commonly used cell types for RA research are primary fibroblast-like synoviocytes (FLS). These have to be freshly isolated from an RA patient and can be used for up to 1-12 passages. They can be frozen at early passage numbers and thawed for later studies. As far as I am aware, there are no good commercially available cell lines that are useful for RA research.
Thanks a lot for the help.
For RA studies, Should I be taking biopsies of synovial joint from patients... If so how can I separate FLS from other cells from the tissue ... do they have a characteristic phenotype differing from Chondrocytes? What conditions I need to maintain for that?
Some of my colleagues suggested MG63 (Osteoblasts) and Chondrocytes... will that help for these studies?
The type of cell you use will depend on the question that you are asking. There are a lot of papers that have protocols for FLS isolation. Most of them consist of mincing the biopsy, doing a collagenase digest, allowing the FLS to adhere to the tissue culture plates. After a few passages, you should have a relatively clean population of FLS, the other cell types will not adhere or proliferate in the flask.
One paper that has an example protocol is: Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes
Good luck
Thank lot Mr. Finn for the timely help!
I think the protocol by Valencia et al. (Cadherin-11 Provides Specific Cellular Adhesion between Fibroblast-like Synoviocytes) should work in our lab. I am actually getting my hands on cell culture ... Hope it works out well!
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