What is the ideal pH range for ointments and creams for topical application?

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Can we change USP method to In House method?

Can we change USP method to In House method for carrying out the assay of any particular dosage form?

10 November 2017 2,743 1 View

What is the effect of injection volume in HPLC?

If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?

03 April 2016 2,373 2 View

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

03 April 2016 844 0 View

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

03 April 2016 3,735 0 View

Is there any material other than caffeine to calibrate the HPLC??

03 April 2016 7,365 4 View

What is the difference between moisture content and water content?

03 April 2016 4,478 4 View

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

03 April 2016 7,469 0 View

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not?

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?

03 April 2016 6,137 1 View

For how many days a mobile phase for HPLC can be stored?

If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...

02 March 2016 8,022 8 View

Which is the best solvent used for washing of HPLC columns and in what concentration?

02 March 2016 9,490 6 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!

26 June 2024 9,093 4 View

Which pH range is allowed for subcutaneous pump administration?

Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...

19 June 2024 5,950 1 View

Ni-NTA protein purification - how to obtain higher yield concentration?

Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...

16 June 2024 5,876 5 View