Can we change USP method to In House method?

Can we change USP method to In House method for carrying out the assay of any particular dosage form?

10 November 2017 2,438 1 View

What is the effect of injection volume in HPLC?

If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?

03 April 2016 2,093 2 View

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

03 April 2016 3,447 0 View

Is there any material other than caffeine to calibrate the HPLC??

Is there any material other than caffeine to calibrate the HPLC??

03 April 2016 7,188 4 View

What is the difference between moisture content and water content?

What is the difference between moisture content and water content?

03 April 2016 3,662 4 View

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not?

03 April 2016 5,900 1 View

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

03 April 2016 7,143 0 View

For how many days a mobile phase for HPLC can be stored?

If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...

02 March 2016 7,805 8 View

Which is the best solvent used for washing of HPLC columns and in what concentration?

02 March 2016 9,166 6 View

Does anyone know of an assay method for Salmeterol Xinafoate and Fluticasone Propionate DPI by HPLC method???

I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...

02 March 2016 1,307 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View