Can we change USP method to In House method?

Can we change USP method to In House method for carrying out the assay of any particular dosage form?

10 November 2017 2,438 1 View

What is the effect of injection volume in HPLC?

If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?

03 April 2016 2,093 2 View

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

03 April 2016 375 0 View

Is there any material other than caffeine to calibrate the HPLC??

Is there any material other than caffeine to calibrate the HPLC??

03 April 2016 7,188 4 View

What is the difference between moisture content and water content?

What is the difference between moisture content and water content?

03 April 2016 3,662 4 View

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not?

03 April 2016 5,900 1 View

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

03 April 2016 7,143 0 View

For how many days a mobile phase for HPLC can be stored?

If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...

02 March 2016 7,805 8 View

Which is the best solvent used for washing of HPLC columns and in what concentration?

02 March 2016 9,166 6 View

Does anyone know of an assay method for Salmeterol Xinafoate and Fluticasone Propionate DPI by HPLC method???

I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...

02 March 2016 1,307 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View