Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?
Can we change USP method to In House method for carrying out the assay of any particular dosage form?
10 November 2017 2,743 1 View
If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?
03 April 2016 2,373 2 View
Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?
03 April 2016 844 0 View
Is there any material other than caffeine to calibrate the HPLC??
03 April 2016 7,365 4 View
What is the difference between moisture content and water content?
03 April 2016 4,478 4 View
Why do we use methanol as a solvent in KF standardisation? Why not other solvent?
03 April 2016 7,469 0 View
What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
03 April 2016 6,137 1 View
If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...
02 March 2016 8,022 8 View
Which is the best solvent used for washing of HPLC columns and in what concentration?
02 March 2016 9,490 6 View
I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...
02 March 2016 1,478 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View