Can we change USP method to In House method?

Can we change USP method to In House method for carrying out the assay of any particular dosage form?

10 November 2017 2,438 1 View

What is the effect of injection volume in HPLC?

If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?

03 April 2016 2,093 2 View

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

03 April 2016 375 0 View

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

03 April 2016 3,447 0 View

Is there any material other than caffeine to calibrate the HPLC??

Is there any material other than caffeine to calibrate the HPLC??

03 April 2016 7,188 4 View

What is the difference between moisture content and water content?

What is the difference between moisture content and water content?

03 April 2016 3,662 4 View

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not?

03 April 2016 5,900 1 View

For how many days a mobile phase for HPLC can be stored?

If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...

02 March 2016 7,805 8 View

Which is the best solvent used for washing of HPLC columns and in what concentration?

02 March 2016 9,166 6 View

Does anyone know of an assay method for Salmeterol Xinafoate and Fluticasone Propionate DPI by HPLC method???

I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...

02 March 2016 1,307 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View