Why do we use methanol as a solvent in KF standardisation? Why not other solvent?
Can we change USP method to In House method for carrying out the assay of any particular dosage form?
10 November 2017 2,743 1 View
If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?
03 April 2016 2,373 2 View
Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?
03 April 2016 844 0 View
Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?
03 April 2016 3,735 0 View
Is there any material other than caffeine to calibrate the HPLC??
03 April 2016 7,365 4 View
What is the difference between moisture content and water content?
03 April 2016 4,478 4 View
What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
03 April 2016 6,137 1 View
If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...
02 March 2016 8,022 8 View
Which is the best solvent used for washing of HPLC columns and in what concentration?
02 March 2016 9,490 6 View
I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...
02 March 2016 1,478 1 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View