What is the effect of injection volume in HPLC?

If we increase or decrease the injection volume in HPLC will it make any difference in the retention time of the peak?

03 April 2016 2,093 2 View

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

Why do we inject a standard 6 times for Related substances by HPLC and 5 times for Assay?

03 April 2016 375 0 View

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

Why do we inject sample twice in ASSAY by HPLC analysis but in RS once?

03 April 2016 3,447 0 View

Is there any material other than caffeine to calibrate the HPLC??

Is there any material other than caffeine to calibrate the HPLC??

03 April 2016 7,188 4 View

What is the difference between moisture content and water content?

What is the difference between moisture content and water content?

03 April 2016 3,662 4 View

What is the function of end capped column in HPLC? How can we confirm the HPLC column is end-capped or not?

03 April 2016 5,900 1 View

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

Why do we use methanol as a solvent in KF standardisation? Why not other solvent?

03 April 2016 7,143 0 View

For how many days a mobile phase for HPLC can be stored?

If any mobile phase contains a mixture of organic solvent and buffer, then for how many days can we store it for using it again and again? Also, if the mobile phase contains only the mixture of...

02 March 2016 7,805 8 View

Which is the best solvent used for washing of HPLC columns and in what concentration?

02 March 2016 9,166 6 View

Does anyone know of an assay method for Salmeterol Xinafoate and Fluticasone Propionate DPI by HPLC method???

I'm trying to develop assay method for Salmeterol and Fluticasone DPI, a product similar to Seroflo rotacaps, CIPLA. Has anyone worked or developed assay method for this product before? Please...

02 March 2016 1,307 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View