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Questions related from Christian Q. Scheckhuber
In a textbook (Biochemistry, 2nd edition by C. Grisham and R. H. Garrett) I found the brief description of so-called calcisomes. These are vesicles/organelles that store calcium, similar to the...
03 March 2018 1,072 2 View
Steroid hormones are usually transported in the blood stream by carrier proteins. At their target cells they are released and can then either bind to surface receptors or diffuse through the...
03 March 2018 5,426 6 View
According to the principle of energy conservation it is not possible to create or destroy energy. Remarkably, PubMed database queries using the terms (mitochondria AND "energy generation") or...
06 June 2016 6,536 17 View
I am curious what your favorite cloning method is and why. Is it Gateway cloning, Gibson assembly, Golden Gate cloning or 'cut and paste' assembly via restriction enzymes and ligase? Or a...
10 October 2015 5,899 2 View
Protease inhibition is essential for the quality of isoelectric focusing/2-D SDS-PAGE experiments. I recently used the 'Protease Inhibitor Cocktail for use with yeast and fungal extracts' from...
03 March 2015 5,817 8 View
I recently tested the components of the "Yeast vacuole staining kit" from Life technologies for staining vacuoles in Penicillium chrysogenum. Carboxy-DCFDA and MDY-64 (both use the GFP...
11 November 2014 9,759 3 View
Cold Spring Harbor Laboratory offers a free pre-print server for the biology field (http://biorxiv.org/), similar to arXiv.org which is a pre-print server for manuscripts in the area of physics,...
10 October 2014 502 7 View
