What is the difference between ESCs and ESCs cell line? Is there any difference in expression of genes and differentiation and survival? What is the point to be titled?
when we use bacteria as a host for produce enzyme and proteins after each bacteria Division ، gene of interest changes and generates a variety of proteins . How can i separate functional and...
03 April 2019 429 3 View
how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .
03 April 2019 3,644 2 View
I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...
03 April 2019 8,048 1 View
After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?
03 April 2019 1,726 4 View
I want lyophilized master mix with enzyme. what is the best lyophilization method in lab with low cost ?
02 March 2019 9,544 2 View
The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...
02 March 2019 5,290 4 View
we tried dissociate blastomere of mice by trypsin but after several protocols I lost my blastomeres and I didnt find them . how can I dissociation blastomeres and generate sin gle cells without...
11 December 2018 5,612 0 View
after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...
02 March 2018 5,791 4 View
I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?
01 February 2018 3,017 2 View
Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ? RdRp related to negative sense RNA viruses
31 December 2017 5,956 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View