How can i separate functional and nonfunctional enzyme or proteins for produce a pure and functional enzyme or protein?

03 April 2019 332 3 View

Time of the GC or TA Melting Temperature?

how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .

03 April 2019 3,571 2 View

Which books or videos or studeis for syntetich biology?

I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...

03 April 2019 7,972 1 View

What is the best way to maintain a plasmid structure without any nucleotide changes?

After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?

03 April 2019 1,619 4 View

What is the best lyophilization method in lab with low cost ?

I want lyophilized master mix with enzyme. what is the best lyophilization method in lab with low cost ?

02 March 2019 9,467 2 View

What is the best way storing master mixes for months ?

The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...

02 March 2019 5,207 4 View

What is the best protocols for blastomere Dissociation ?

we tried dissociate blastomere of mice by trypsin but after several protocols I lost my blastomeres and I didnt find them . how can I dissociation blastomeres and generate sin gle cells without...

11 December 2018 5,534 0 View

After replication of RNA virus in host how virus detect RNA genome from host's RNA?

after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...

02 March 2018 5,715 4 View

What is the best dyes for finding several viruses in a sample for use in Real Time PCR?

I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?

01 February 2018 2,947 2 View

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ?

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ? RdRp related to negative sense RNA viruses

31 December 2017 5,884 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View