How can i separate functional and nonfunctional enzyme or proteins for produce a pure and functional enzyme or protein?

03 April 2019 332 3 View

Time of the GC or TA Melting Temperature?

how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .

03 April 2019 3,571 2 View

Which books or videos or studeis for syntetich biology?

I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...

03 April 2019 7,972 1 View

What is the best way to maintain a plasmid structure without any nucleotide changes?

After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?

03 April 2019 1,619 4 View

What is the best lyophilization method in lab with low cost ?

I want lyophilized master mix with enzyme. what is the best lyophilization method in lab with low cost ?

02 March 2019 9,467 2 View

What is the best way storing master mixes for months ?

The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...

02 March 2019 5,207 4 View

After replication of RNA virus in host how virus detect RNA genome from host's RNA?

after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...

02 March 2018 5,715 4 View

What is the difference between ESCs and ESCs cell line?

What is the difference between ESCs and ESCs cell line? Is there any difference in expression of genes and differentiation and survival? What is the point to be titled?

01 February 2018 7,052 0 View

What is the best dyes for finding several viruses in a sample for use in Real Time PCR?

I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?

01 February 2018 2,947 2 View

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ?

Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ? RdRp related to negative sense RNA viruses

31 December 2017 5,884 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View