we tried dissociate blastomere of mice by trypsin but after several protocols I lost my blastomeres and I didnt find them . how can I dissociation blastomeres and generate sin
gle cells without lost them?
when we use bacteria as a host for produce enzyme and proteins after each bacteria Division ، gene of interest changes and generates a variety of proteins . How can i separate functional and...
03 April 2019 429 3 View
how we can calculate Melting Temperature time for GC and TA and a primer ? I want time of this process for each GC and AT and our primers .
03 April 2019 3,644 2 View
I am very interested in the field of synthetic biology and I intend to begin studies on this subject. But I cant find the right way, How do I do my research that does not eliminate my...
03 April 2019 8,048 1 View
After each replication of the plasmid in bacteria, it undergoes nucleotide changes. What is the best way to maintain a plasmid structure without any nucleotide changes ?
03 April 2019 1,726 4 View
I want lyophilized master mix with enzyme. what is the best lyophilization method in lab with low cost ?
02 March 2019 9,544 2 View
The problem with freezing our mastermixs with the enzyme is that freezing certain active enzymes such as the polymerase family of enzymes tends to result in diminished or ablated enzyme...
02 March 2019 5,290 4 View
after replication of RNA viruses in the cytoplasm of the host, both of genome RNA and host RNA are existing in the cytoplasm but for packing of the virus , it should detect its RNA genome. How is...
02 March 2018 5,791 4 View
What is the difference between ESCs and ESCs cell line? Is there any difference in expression of genes and differentiation and survival? What is the point to be titled?
01 February 2018 7,128 0 View
I want to detect several viruses using Real-Time PCR simultaneously. What is the best probes and dyes attached to them?
01 February 2018 3,017 2 View
Are there Any RdRp( Rna dependent Rna polymerase) with repair ability and without mutagenesis ? RdRp related to negative sense RNA viruses
31 December 2017 5,956 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View